For research use only. Not for use in diagnostic procedures.

Answer

The Bio Tool Kit microapp embedded in PeakView? or SCIEX OS Software cannot perform de novo manual sequencing of peptides or MS/MS fragment matching for peptides fragmented using electron activated dissociation (EAD) from a ZenoTOF 7600 system. The MS/MS data obtained by EAD is too complex and fragment-rich, presenting the software with too many options for potential fragment ions; however, the Bio Tool Kit app can process MS/MS data acquired by CID (collision-induced dissociation). These EAD-based MS/MS spectra can be automatically interpreted and annotated by Biologics Explorer software if the sequences are already known. To process de novo sequencing data, software from third-party vendors, such as Peaks software, is required.

For peptide MS/MS data acquired by CID, users can follow three steps to perform?de novo sequencing using Bio Tool Kit:?

1. Highlight a fragment ion as a starting point for manual sequencing.
2. Double-click on the caption for the peak to characterize it.
3. View the peptide sequence ladder to obtain the final results.

Analysis of peptide fragment MS/MS spectra using Bio Tool Kit has been described elsewhere:
1) In this article: https://sciex.com/support/knowledge-base-articles/how-to-use-bio-tool-kit-for-interpreting-peptide-ms-ms-data-acquired-with-cid-and-ead-fragmentation_en_us
2) In this SCIEX community post: https://community.sciex.com/2021/08/07/using-bio-tool-kit-for-interpreting-peptide-ms-ms-data-obtained-using-electron-activated-dissociation-ead/

?

?

?